生物物理新技术方法在蛋白结构与功能中的应用研究

Application of New Physical Techniques and Methods to the Structure and Function of Proteins

现代物理技术与方法的发展为蛋白分子的研究提供了丰富而有效的手段。首先强调了技术与科学发展的辩证关系;介绍了在蛋白分子研究中的常用物理技术与方法如质谱技术、核磁共振技术、同步辐射X射线衍射技术、差示扫描量热技术的物理原理及其在大分子研究中的应用现状,并重点介绍了一些新兴的非常规研究手段诸如原子力显微镜、激光光镊技术的特点及其在蛋白研究中的最新应用和研究成果;通过对比诸多技术与方法近年来在国内外研究中的进展情况,对各种技术与方法的优缺点进行了阐述。

The developing modern physical techniques offer a series of abundant and effective methods to the study on proteins instructure and function.Firstly we stressed on the dialectic relationship between physical techniques and the improvement of science inhistory.And then introduced a lot of physical techniques in common use in proteins such as mass spectroscope,nuclear magneticresonance,synchronization X-ray diffraction,and differential scanning thermal analysis,and reviewed their application status in quo toproteins.Secondly paid the attention to the burgeoning unconventionally instruments i.e.atomic force microscope and laser opticaltweezers which have attracted the professional men in concern,and introduced latest application and researches’ fruits.Compared thedevelopment of techniques in recent years,have set forth the shortcoming and excellence of all kinds of equipments introduced.Andended with the conclusion that it is necessary to manage the possible instruments effectively and sufficient for the personalities,and givenout the optimum research routes which emphasized on the new techniques and fresh methods i.e.atomic force microscope and opticaltweezers.