实验性脑出血急性期灶周组织水肿及炎性细胞因子的表达

The expression of inflammatory cytokines around the hematoma after intracerebral hemorrhage

目的研究脑出血血肿周围脑组织炎性细胞因子的表达,探索其动态变化规律及与脑水肿的关系。方法采用Wistar大鼠脑内缓慢注入自体血的方法建立实验性脑出血动物模型;随机分为6组,测定实验性脑出血6h、12h、24h、3d、5d、7d时间点血肿周围脑组织的含水量;应用免疫组化法及免疫印迹法(Western Blot)观察血肿周围脑组织中细胞因子IL-6、TNFα的表达。结果脑出血后血肿周围脑组织的含水量逐渐增加,于3d达高峰;免疫组化染色显示血肿周围组织中神经细胞、血管内皮细胞均有IL-6及TNFα的阳性表达。免疫印迹半定量分析显示脑出血后6h血肿周围脑组织中即可见IL-6、TNFα较高水平的表达,24h达高峰,此后逐渐下降。结论脑出血急性期(6h)IL-6及TNFα参与了血肿周围脑组织的损伤过程。IL-6变化曲线与脑水肿曲线呈现出一致性,推测其参与了血肿周围水肿的形成。

Objective To study the expression of inflammatory cytokines around the hemotoma after intracerebral hemorrhage(ICH) and to explore the relationship between the cerebral edema and the inflammatory cytokines. Methods Experimental ICH in rats were induced by autologous nonheparinized blood injection. Water content was measured at 6h,12h,24h,3d,5d and 7d by dry-wet weight. The expression of IL-6 and TNF-αaround the hemotoma was observed by immunohistochemistry and Western Blot. Results The intracranial edema reach peak on 3d. The expression of IL-6 and TNF-α were observed in the 6h group by Western Blot,and reached peak in the 24h group,then decreased gradually. The positive expression of IL-6 and TNF-αwere detected in nerve cells and vascular endothelial cells around the hemotoma by immunohistochemistry stain. Conclusion IL-6 and TNF-α involve in the injury formation of peri-hemotoma during the acute period of intracerebral hemorrhage. Since there is positive correlation between the IL-6 and cerebral edema curve,we speculate that there is positive correlation between IL-6 and the cerebral edema around the hemotoma.