高效液相色谱法测定红霉素眼膏的含量

Determination of Erythromycin in Erythromycin Eye Ointment by HPLC

目的建立高效液相色谱法测定红霉素眼膏的含量。方法采用十八烷基硅烷键合硅胶(5um)为分析柱。流动相:0.1mol/L磷酸二氢铵溶液(三乙胺调节pH6.5)–乙腈(70:30);流速:1.0ml/min;检测波长210nm。进样量20ul。结果在进样量2.5～80.0ug的范围内,进样量与峰面积线性关系良好(r=0.9999),重复进样RSD=0.31%。结论该方法简便快速,重现性好,可作为红霉素眼膏的含量测定方法。

Objective To establish an HPLC method for the determination of erythromycin in erythromycin eye ointment.Methods The analytical column was ODS C18(250×4.6 mm,5μm), the mobile phase was composed of 0.1M NH4H2PO4 and methanol(70:30, v/v) with the flow rate of 1.0 mL·min-1, the detection wavelength was 210nm and the column temperature was 30℃. External standard method was used.Results The Calibration curve was linear within the concentration range of 2.5~80.0 ug. The average recovery was 99.3% ,RSD was 0.31%(n=3).Conclusion The method is simple, rapid and accurate. It is suitable for the determination of erythromycin in erythromycin eye ointment.