利用代谢产物检测华山松疱锈病的初步探讨

Preliminary Studies on Detecting Blister Rust by Metabolites of Pinus armandii

以感染疱锈病的华山松树皮、锈孢子及健康华山松的树皮为材料,用甲醇溶液提取其代谢产物,通过紫外扫描法、薄层层析法和高效液相法检测代谢产物的差异.结果表明:华山松疱锈菌孢子甲醇提取代谢物在紫外区的214,307 nm处有两个最大的吸收峰,而树皮代谢产物最大吸收值不明显;在307 nm紫外检测中,感病株树皮和锈孢子代谢产物有一相同的组分,此组分在健康株树皮中没有检测出.乙酸乙酯:乙醇=15∶1能较好将感病株和健康株树皮区分.高效液相法显示在214 nm波长处检测可将感病株和健康株区分,同时也证明感病株树皮和锈孢子的代谢产物在保留时间为10.30～10.40 min有相同的峰,说明感病株树皮和锈孢子具有相同的代谢产物.

Taking uredospores of Cronartium ribicola,the Pinus armandii bark infected byCronartium ribicola and the healthy bark as experimental materials,the differences in metabolites extracted with methanol were analyzed by means of UV scanning,TLC and HPLC.The results indicated that the metabolite extracted from uredospores of Cronartium ribicola had two strong absorptive peaks at 214 nm and 307 nm wave lengths,but there were no evident peak values absorbed by the metabolite of tree barks.It was showed by UV scanning at 307 nm wave length that there was a component commonly detected in metabolites extracted from both the infected bark and the uredospores,which was not found out in the metabolite extracted from the healthy bark.Solvent system(ethyl acetate ∶ethanol=15 ∶1)could differentiate the metabolites extracted from the infected and the healthy barks of Pinus armandii.HPLC detected results also showed that the metabolites extracted from both the uredospores of Cronartium ribicola and the infected bark had similar absorption peak at 214 nm wave length while the retaining time was from 10.30 min to 10.40 min,but the metabolite extracted from the healthy bark did not have this special peak.The above resulted showed the possibility of telling the difference of the infected plants from the healthy ones.