无花果果浆对肿瘤细胞增殖抑制和诱导凋亡作用

Proliferation Inhibition and Apoptosis Induction of Fig Fruit Latex on Human Cancer Cells

为了探讨无花果果浆(Fig fruit latex,FFL)对人肿瘤细胞的生长抑制作用及其机制,用无花果果浆处理体外培养的人肿瘤细胞,细胞增殖试验(MTT法)、克隆形成试验研究FFL对人肿瘤细胞的增殖抑制作用,Brdu掺入试验、吖啶橙/溴乙啶(AO/EB)染色、流式细胞术检测FFL对肿瘤细胞DNA合成、凋亡和细胞周期的影响。结果,用FFL处理后,肿瘤细胞增殖活性降低(P<0.05),克隆形成下降(P<0.05),Brdu标记指数降低(P<0.05),吖啶橙染色凋亡细胞增多(P<0.05);细胞周期分布改变,凋亡指数升高(P<0.01),G0/G1期细胞数增加(P<0.01),S期细胞数减少(P<0.01),在一定剂量内对正常细胞无明显影响。试验结果提示,FFL对所试肿瘤细胞的增殖有显著地抑制作用,其作用机理可能与抑制肿瘤细胞DNA合成,诱导肿瘤细胞凋亡及细胞周期阻滞有关。

To investigate the effect of fig fruit latex(FFL) on the proliferation of human cancer cells and to elucidate the mechanism of its activity,human glioblastoma,hepatocellular carcinoma and normal liver cells were tested in vitro for cytotoxicity,colony formation inhibition,Brdu incorporation,AO/EB apoptotic cell staining,and cell cycle distribution through flow cytometry after treatment with FFL.After FFL treatment,the proliferation,colony formation and Brdu labeling indices of cancer cells decreased(P<0.05);AO/EB stained apoptotic cells increased(P<0.05).By FCM analysis,the apoptotic indices increased(P<0.01),the cells increased in G0/G1 phase(P<0.01)and decreased in S phase(P<0.01).The findings in this experimental study suggest that FFL has cytotoxicity on some human cancer cells,but little effect on normal cells at certain concentration,and its mechanism of activity might be associated with the inhibition of DNA synthesis of cancer cells,induction apoptosis and cell cycle arrest of cancer cells.