由环己酮生物合成己内酯的研究

Study on Biosynthesis of Caprolactone from Cyclohexanone

从本实验室保藏菌种中分离筛选出一株能够以环己酮作为唯一碳源的菌株,初步鉴定为邻单胞菌属,并对其产物进行GC/MS定性,发现有己内酯生成,存在Baeyer-villiger反应。文章还探讨了pH,装液量,底物浓度,培养时间,温度以及转速等条件对细菌生长的影响,并进一步研究了细菌的底物广谱性,发现此菌能够利用与环己酮具有相似结构的环戊酮等有机物。

A strain which can utilize cyclohexanone as sole carbon source was isolated and identified as Plesiomonas sp.,and in its productions,caprolactone was found with GC/MS and Baeyer-villager reaction also existed.Effects of pH,volume,concentration of cyclohexanone,cultivating time,temperature and rotate speed on the growth of bacteria were discussed,and the other organic substrates were also studied.The results indicate that the strain can utilize these organic substrates having the similar structure with cyclohexanone such as cyclopentanone etc.