摘要
以总黄酮为考察指标,确定纤维素酶提取红景天有效成分的最佳工艺。研究酶解条件对总黄酮浸出率的影响,并对不同提取方法的提取效果和粗提物的DPPH清除活性进行了比较。纤维素酶提取法的最佳工艺条件为:加酶量为1.95%(以原料干重计),液料比为70:1(mL:g),pH值为5.5,酶解温度为40℃,酶解时间为5 h,红景天总黄酮的浸出率为4.385%。酶解技术可明显提高红景天总黄酮的浸出率,粗提物得率高且DPPH清除活性较强。
To study the process for extracting total flavones from Rhodiola sachalinensis by cellulase,and the relationship between enzymatic conditions and the leaching rate of total flavones,the extraction effects of different extraction methods and the DPPH radical scavenging activities of the resulted crude extracts were investigated.The optimum condition for extraction with cellulase is as follows:the amount of cellulase,1.95%,the liquid-solid ratio,70:1(mL:g),pH,5.5,the temperature of enzyme digestion,40 ℃,and the extracting time,5 h.The leaching rate of total flavones is up to 4.385%.The crude extract obtained by cellulase method has the highest yield and higher DPPH radical scavenging activity.
出处
《天然产物研究与开发》
CAS
CSCD
2006年第5期818-821,共4页
Natural Product Research and Development
基金
国家自然科学基金项目(30472135)
关键词
红景天
纤维素酶
黄酮
酶解工艺
DPPH清除活性
Rhodiola sachalinensis
cellulase
flavones
enzymatic process
DPPH radical scavenging activity
