丝裂霉素对碱烧伤后角膜新生血管的抑制作用

The effect of Mitomycin C applied locally in Corneal Neovascularization of rabbit after alkali burning

目的:探讨丝裂霉素抑制角膜新生血管的作用。方法:36只家兔分成三组,每组12只,右眼碱烧伤制成新生血管模型,A组给予生理盐水点眼,B组给予丝裂霉素结膜下注射,C组给予地塞米松眼液点眼,隔日观测角膜情况,于7d、14d各取半数家兔处死,取角膜组织行HE染色及VEGF免疫组化检测。结果:丝裂霉素对角膜新生血管有抑制作用(差异有显著性),比地塞米松作用稍强(差异无显著性),后期反应较其轻。结论:非细胞毒浓度丝裂霉素具有明显抑制新生血管的作用。

Objective: To evaluate the effect of mitomycin-C on inhibiting corneal neovascularization.Methods:The corneal neovascularization was induced on the right eyes by alkali burn in 36 white rabbits.The rabbits were randomly divided into 3 groups,12 rabbits in each group.0.9% Sodium chloride was dropped four times daily in group A,mitomycin-C was injected subconjunctival tissues in group B,dexamethasone was dropped four times daily in group C.The development of corneas were observed,the cornea tissues were accounted by immunhistochemical analysis of Vascular Endothelial Growth Factor.Results:The corneal neovascularization were inhibited strongly in group B than it in group A and C,and the immunreactivity were slower and fewer.Conclusion:Topical application of mitomycin-C has significant effects on the inhibition of corneal neovascularization.