摘要
从侧孢短芽孢杆菌X10发酵液中提取的拮抗蛋白粗提液具有抑制茄科劳尔氏菌生长的效果,该抗菌蛋白粗提液具有良好的热稳定性,能耐90℃的高温;用蛋白酶处理,其抑菌活性受胰蛋白酶、胃蛋白酶和蛋白酶K的影响;对氯仿敏感;随着紫外照射时间的延长,活性受到影响;拮抗物质25℃保存,活性28天内基本不变;作用活性pH值(pH值5.0～11.0)范围较宽.
The crude protein extracted from the Brevibacillus laterosporus X10 culture could inhibit the growth of Ralstonia solanacearum.The crude protein was thermal stable,which could tolerate 90℃.The inhibitory activity was influenced by treatment with trypsin,pepsin and proteinase K.It was sensitive to chloroform.Along with the time of ultraviolet irradiation extending,the inhibitory activity was reduced.The antimicrobial protein could keep its antibacterial activity stable for 28d at 25℃.The active pH range of the crude protein to the pathogen was wide,from 5.0 to 11.0.
出处
《生物学杂志》
CAS
CSCD
2006年第5期16-19,共4页
Journal of Biology
基金
国家高技术研究发展计划项目(863计划)
项目编号:NO2002AA245031
关键词
侧孢短芽孢杆菌
拮抗物质
提取
抑菌活性
Brevibacillus laterosporus
antagonistic substance
isolation
inhibitory activity
