摘要
以黑籽南瓜和白籽南瓜为试材,研究了苗龄、激素配比、外植体、芽诱导时间以及基因型对再生频率的影响.结果表明,以3～5天苗龄的南瓜子叶为外植体,在MS+6-BA 1 mg/L+Ades 20 mg/L的诱导培养基上诱导10天后,转入MS+GA30.5 mg/L的芽伸长培养基,不同基因型南瓜均能获得较高的植株再生率,每个外植体成苗数在2.5个以上.从接种到再生苗移栽仅需4～5周时间.
Effects of seedling age,the hormone combination,different explants,the uime of shoot induction and different genotypes on pumpkin tissue culture efficiency were studied.The results showed that: when the pumpkin cotyledons from 3～5 days seedling were induced in the medium MS+6-BA 1 mg/L+Ades 20 mg/L for about 10 days,and then transferred into shoot elongation medium,MS+GA_3 0.5 mg/L,more plantlets could regenerate rapidly from different genotype explants.The time from cotyledon subculturing to plantlet transplanting needed only 4～5 weeks.
出处
《山东农业科学》
2006年第6期4-6,共3页
Shandong Agricultural Sciences
基金
山东省"三○"工程资助项目
关键词
南瓜
子叶
外植体
植株再生
Pumpkin
Cotyledon
Explants
Plantlet regeneration
