补阳还五汤及有效部位生物碱和苷对动脉血栓形成大鼠血浆血栓素A_2、前列环素的影响

Effect of Buyang Huanwu Decoction and Its Active Fractions Such as Alkaloid and Glycoside on TXA_2 and PGI_2 of Arterial Thrombosis Model in Rats

目的:探讨补阳还五汤及其有效部位生物碱和苷对大鼠动脉血栓形成及血浆血栓素A2(TXA2)和前列环素(PGI2)的影响。方法:对颈总动脉血栓模型大鼠分别给予补阳还五汤原方、生物碱、苷、低分子肝素和抵克利得,比较各组动脉血栓重量和血浆TXB26、-keto-PGF1α的变化。结果:模型组可形成闭塞性血栓,TXB2升高(P<0.05),6-kcto-PGF1α降低(P<0.05)。各药均可使血栓重量减轻。原方可使6-keto-PGF1α升高(P<0.05),而对TXB2无显著影响。生物碱可降低TXB2(P<0.05),升高6-keto-PGF1α(P<0.05)。苷、低分子肝素和抵克利得对TXB2和6-keto-PGF1α均无显著影响。结论:补阳还五汤原方、生物碱、苷、低分子肝素和抵克利得均有抗血栓作用。原方的作用与促进PGI2合成有关;生物碱的作用与抑制TXA2合成、促进PGI2合成有关。而苷、低分子肝素和抵克利得不是通过调节TXA2和PGI2合成发挥作用的。

Objective:To explore effect of Buyang Huanwu Decoction(BHD) and its active fractions such as alkaloid and glycoside extracted from BHD on thromboxane(TXA_2) and prostacyclin(PGI_2) of arterial thrombosis model in rats.Methods:Carotid arterial thrombosis model in rats was induced by topical ferric chloride.The model rats were administered with BHD,alkaloid,glycoside,low molecular weight heparin(LMWH) and ticlopidine respectively.Weight of thrombus and the levels of TXB_2 and 6-keto-PGF_(1α) in plasma in each group were compared.Results:Occlusive thrombus was induced,the levels of TXB_2 were increased markedly(P<0.05),the levels of 6-ketoPGF_(1α) were lower remarkably in the model group(P<0.05).Weight of thrombus in BHD,alkaloid,LMWH and ticlopidine group was significantly lower than that of the model group(P<0.05).BHD could increase the levels of 6-ketoPGF_(1α)(P<0.05),but it did not influence the levels of TXB_2.Alkaloid could decrease the levels of TXB_2 and increase the levels of 6-keto-PGF_(1α) significantly(P<0.05).Glycocide,LMWH and ticlopidine had no influence on the levels of TXB_2 and 6-keto-PGF_(1α).Conclusions:Antithrombotic actions of BHD,alkaloid,glycoside,LMWH and ticlopidine were observed.Antithrombotic actions of BHD may be related to accelerating synthesis of PGI_2.Antithrombotic actions of alkaloid may be associated to inhibiting production of TXA_2 and increasing synthesis of PGI_2.Antithrombotic actions of glycoside,LMWH and ticlopidine was not based on its modulation on TXA_2 and PGI_2,it may be concerned with other aspects.