摘要
目的:观察垂盆草小麦黄素苷对小鼠脾脏T、B淋巴细胞转化增殖的影响。方法:采用小鼠脾细胞3H-TdR掺入法,用刀豆球蛋白(ConA)或脂多糖(LPS)刺激,测定掺入细胞DNA中3H-TdR的放射性相对数量(cpm)。结果:小麦黄素苷100,10,1μg/mL(终浓度)对T淋巴细胞的cpm数有非常显著的抑制作用(均P<0.001);小麦黄素苷100μg/mL对B淋巴细胞的cpm数有非常显著的抑制作用(P<0.01)。结论:垂盆草小麦黄素苷能明显抑制正常小鼠脾脏T、B淋巴细胞增殖。
Objective:To observe the influence of sedum sarmentosum tricin-glucoside on proliferation of spleen T and B lymphocytes of normal mice.Methods:Method of mixturing()~3H-TdR to mice spteen cell was applied.After stimulation with canavaline(ConA)or lipid polysaccharides(LPS),the radioactive relative quantity(cpm) of()~3H-TdR in DNA in spleen cell.Results:The dosages in 100,10,1μg/mL(final concentration)of sedum sarmentosum tricin-ghcoside had highly significant inhibition to cpm number of T lymphocyte(P<0.001);the dosage in 100μg/mL of sedum sarmentosum tricin-glucoside had highly significant inhibition to cpm number of B lymphocyte (P<0.01).Conchsions:Sedum sarmentosum tricin-glucoside can obviously inhibit proliferation of spleen T and B lymphocytes of normal mice.
出处
《中国实验方剂学杂志》
CAS
2006年第10期29-31,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部科研课题(99-929-01-34)
关键词
垂盆草小麦黄素苷
免疫
抑制
Sedum sarmentosum tricin-glucoside:Immunity
Inhibition