加味鳖甲煎丸对白蛋白所致大鼠免疫性肝纤维化的防治作用

The Preventive and Therapeutic Effect of Jiaweibiejiajian Pill on Rats with Hepatic Fibrosis Induced by Serum Albumin

目的:观察加味鳖甲煎丸对白蛋白所致大鼠免疫性肝纤维化的防治作用。方法:用人血清白蛋白给大鼠皮下注射免疫4次,在大鼠明显产生抗体后分组给药,再由尾静脉注射人血清白蛋白9次,可得到肝纤维化实验动物模型,实验结束后处死大鼠,收集血清,测定血清AST、ALT、TP、ALB、TBIL、A/C和CIV、LN、HA的含量。结果:同模型组相比,加味鳖甲煎丸能显著降低肝纤维化大鼠血清AST、ALT、TBIL、CIV、LN、HA含量;能显著增加TP、ALB含量和A/G比值,能显著改善大鼠肝纤维化程度。结论:加味鳖甲煎丸对白蛋白所致大鼠免疫性肝纤维化有较好的防治作用。

Objective:To study the preventive and therapeutic effect of Chinese medicine Jiaweibiejiajian Pill(JWBJ)on rats with hepatic fibrosis induced by human serum albumin.Methods:The rats model with hepatic fibrosis was induced by multiple subcutaneous injections of serum albumin solution for 4 times.After it was confirmed that there were albumin antibody in rats serum,and then rats were divided into model-group,low-dose group of JWBJ,mediate-dose group of JWBJ and high-dose group of JWBJ.Rats were given JWBJ orally every day,and injected albumin solution into the rats caudal veins for 9 times during 4 weeks.When hepatic fibrosis was verified to come into being by pathological examination.Alanine amino transaminase(ALT),aspartate aminotranferase(AST),total protein(TP),albumin(ALB) Hyaluronic acid(HA),Laminin(LN),Collagen Type(PC) in serum of all rats were examined.Results:Compared with the model group,JWBJ could obviously decrease the concentration of serum ALT,AST,HA,LN,CIV and increase the concentration of serum ALB,TP,and increase the ratio of serum A/G of rats with hepatic fibrosis,and reduce the degree of hepatic fibrosis.Conclusion:JWBJ can effectively prevent and treat hepatic fibrosis induced by serum albumin.