健脾与补肾对脑内精氨酸加压素水平和基因表达的影响

The Influence of Invigorating Spleen and Restoring Virility on the Arginine Vasopressin Level in Brain and Gene Expression

目的:比较健脾与补肾对脑内精氨酸加压素(AVP)水平和基因表达的影响。方法:采用苦降泻下、饮食失节加劳倦过度法建立脾虚大鼠模型,以免疫组化和原位杂交法检测下丘脑腹侧核、海马CA1区、前额叶皮层精氨酸加压素(AVP)基因表达变化。结果:模型组上述脑区AVP免疫阳性反应物明显降低;健脾组与补肾组上述脑区的AVP免疫阳性反应物明显升高。模型组下丘脑腹侧核、海马CA1区、前额叶皮层AVP mRNA表达显著下降;健脾组与补肾组AVP mRNA表达明显升高。结论:脾虚模型脑内对学习记忆有促进作用的AVP水平和基因表达有变化,健脾与补肾可能通过调节AVP水平和基因表达而影响学习记忆能力。

Objective:To Study the influence of invigorating spleen and restoring virility to the AVP level in brain and gene expression.Method:Establishing the spleen insufficiency model of rat by adopting bitter decline diarrhea,wild eating and overworking,the hypothalamus ventral kernel,the hippocampi CA1 area and forehead cortex,the gene expression change of AVP were examined.Result:In the model group,the immunoreaction masculine substance of AVP,hypothalamus ventral kernel,hippocampi CA1 area and prefrontal cortex decreases obviously;The immunoreaction masculine substance of AVP on above mentioned parts in the group of invigorating spleen and restoring virility increases distinctly.In the model group,the expression of AVP mRNA on hypothalamus ventral kernel,hippocampi CA1 area and prefrontal cortex decreases obviously;While for the groups of invigorating spleen and restoring virility,they increases obviously.Conclusion:Invigorating spleen and restoring virility may affect study and memory ability by regulating the AVP level and gene expression.