摘要
目的:为了阐明基于药物代谢酶的中药十八反产生相反作用的机制,选择相反药对甘草、芫花作为研究对象,研究二者合用对主要药物代谢酶CYP1A2、CYP2E1、CYP3A1/2酶活性的影响.方法:采用高效液相色谱法测定CYP1A2、CYP2E1活性;采用紫外-可见分光光度法测定CYP3A1/2活性.结果:甘草、芫花合用后CYP1A2、CYP2E1、CYP3A1/2的酶活性增加,与对照组比较有显著差异(P＜0.05～0.001).结论:两药合用对药物代谢酶的影响可能会使某些药物毒性成分在体内代谢特征发生改变,对药物的疗效或毒性产生影响,从而产生基于药物代谢酶的中药间相互作用.
Objective:To elucidate the imcompatible mechanism of traditional Chinese Medicine,the effect of Aconitum coadministration with Lilac Daphne on the main enzyme activity,such as CYP1A2,CYP2E1 and CYP3A1/2.Methods:CYP1A2 and CYP2E1 activities were quantitated by high performance liquid chromatographic(HPLC) assay;CYP3A1/2 activity was quantitated by UV chromatography.Results:Aconitum coadministration with Lilac Daphne obviously promoted the activities of CYP1A2,CYP2E1 and CYP3A1/2(P<0.05～0.001).Conclusion:The effect on the enzymes activity of CYP1A2、CYP2E1、CYP3A1/2 of Aconitum coadministration with Lilac Daphne may change the toxic component's metabolism and influence phamarceutical effect and toxicity.The herb-herb interaction maybe occur.
出处
《中国实验方剂学杂志》
CAS
2006年第12期48-50,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
中药十八反
细胞色素P450
酶活性
eighteen imcompatible pairs
cytochrome P450
enzyme activity
