黄芪对大鼠肾缺血再灌注过程中脂质过氧化损伤的保护作用研究

The Study on Huangqi’s Protective Role of Lipid Peroxidation Injury during Renal Ischemia Reperfusion in Rats

目的探讨黄芪在急性肾缺血再灌注损伤的保护作用机制。方法采用大鼠双侧肾蒂夹闭的肾缺血再灌注损伤模型,分别测定用黄芪组与未用黄芪组肾组织中丙二醛(MDA)水平和超氧化物歧化酶(SOD)的活性。光镜下观察肾组织病理变化。结果肾缺血再灌注组SOD及用药组SOD均比假手术组SOD活力下降,但用药组比单纯肾缺血再灌注组下降少。肾缺血再灌注组中MDA及用药组的MDA值均比假手术组增加,但用药组比单纯肾缺血再灌注组升高少一些。结论黄芪在肾缺血再灌注损伤的脂质过氧化过程中有保护作用。

Objective To investigate the huangqi’s protective of lipid peroxidation injury during reperfusion in renal ischemic rats.Methods The model of rats acute renal ischemic/reperfusion injury was established,and pedicle for 60 minutes was used in this experiment,and Huang Qi was used on the rat renal ischemia reperfusion models.Maleic Dialdehyde (MDA) levels and Superoxide Dismutase (SOD) activities were determined to assess lipid peroxidation.Pathologic changes were studied by optic microscopy. Results The renal content of MDA in ischemia/reperfusion groups was increased, MDA of Huang Qi group were significantly lower than those of ischemia/reperfusion groups. The activity of SOD in renal tissue decreased both in Huang Qi group and ischemia/reperfusion, but SOD of Huang Qi group were significantly higher than those of ischemia/reperfusion. Conclusions Huang Qi has a protective effect on lipid peroxidation injury during reperfusion in renal ischemia rats.