人非小细胞肺癌组织中环氧化酶-2mRNA的表达及临床意义

The Expression and Clinical Significance of Cyclooxygenase-2 mRNA in Human Non-small Cell Lung Cancer

目的检测环氧化酶-2(Cyclooxygenase-2,COX-2)mRNA在人非小细胞肺癌、癌旁及正常肺组织中的表达情况,探讨COX-2mRNA的表达在非小细胞肺癌发生、发展中的意义。方法应用逆转录-聚合酶链反应(RT-PCR)技术检测20例非小细胞肺癌、癌旁及正常肺组织中COX-2mRNA表达水平。结果COX-2mRNA在非小细胞肺癌组织中的表达水平为(1.412±0.375),高于其在癌旁组织中的(0.595±0.296)和正常肺组织中的(0.477±0.105),差异具有显著性(P<0.01);COX-2mRNA表达与患者性别、年龄及肿瘤大小、生长部位均无显著性相关(P>0.05),而与肿瘤组织学分型、分化程度、临床分期及是否淋巴结转移密切相关(P<0.01或P<0.05)。结论COX-2mRNA表达水平在人非小细胞肺癌组织中增高,并与非小细胞肺癌淋巴结转移、远处转移、临床分期等临床病理特征密切相关,COX-2mRNA表达在非小细胞肺癌发生、发展、转移过程中发挥重要作用。

Objective To detect the expression of cyclooxygenase-2 mRNA(COX-2 mRNA)in human non-small cell lung cancer(NSCLC),Para cancerous and normal lung tissues.We To investigate the significance of COX-2 mRNA in the occurrence and development of NSCLC.Methods RT-PCR method were used to investigate the expression of COX-2 mRNA in 20 cases of NSCLC 、Para cancerous and normal lung tissues.Results The expression level of COX-2 mRNA of NSCLC tissues was (1.412±0.375),it was higher than that of Para cancerous tissues(0.595±0.296)and normal lung tissues(0.477±0.105),it was significantly different(P<0.01);The expression level of COX-2 mRNA was related to the tumor pathological type,histological class,clinical stage or metastasis lymph node(P<0.01orP<0.05),but was not related to patients’ age and sex,the size and place of tumor(P>0.05).Conclusions The expression level of COX-2 mRNA of NSCLC tissues was higher,it was related to the tumor pathological type,histological class,clinical stage or mestastatic lymph node,The expression of COX-2 mRNA plays a very important role in the occurrence, development and lymph node metastasis of NSCLC.