摘要
目的采用HPLC-ELSD法测定芪红胶囊中的黄芪甲苷。方法采用Capcell PAK C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(75:25),检测温度115℃,载气流速2.5 ml.min-1,柱温35℃。结果黄芪甲苷能与相邻峰完全分开,进样量1.012~5.06μg与峰面积积分值的线性关系良好(r=0.9998)。结论所建方法简便易行,准确可靠,可用于芪红胶囊中黄芪甲苷的质量控制。
OBJECTIVE To establish an HPLC-ELSD method for the determination of astragaloside Ⅳ in Qihong capsule.METHODSThe HPLC system was consisted of Capcell PAK C18 column(250 mm×4.6 mm,5 μm),a mixture of methanol and water(75:25)as the mobile phase.The detector temperature at 115℃,the flow rate was 2.5 ml·min-1 and the column temperature was set at 35℃.RESULTSThe peak of astragaloside Ⅳ could be separated from others completely,and the linear range of astragaloside Ⅳ was 1.012-5.06 μg(r=0.9998).CONCLUSIONThe esta...
出处
《华西药学杂志》
CAS
CSCD
北大核心
2008年第2期209-210,共2页
West China Journal of Pharmaceutical Sciences
基金
国家自然科学基金资助项目(批准号:30640080)
