半导体激光和紫外线对辅酶Q_(10)产生菌的复合诱变效应

Mutagenic effect on a strain producing CoQ_(10) by cooperation of UV and LD-Laser

目的选育辅酶Q10(CoQ10)高产菌株。方法采用紫外线、半导体激光及紫外线与半导体激光复合作用的方法,诱变产CoQ10红酵母菌SY-3,以提高CoQ10的产量。结果紫外线和半导体激光单独作用,诱变效果不佳;二者合用的效果却很好。用紫外照射120 s,再经半导体激光辐射8 min,得到一株叠氮钠和维生素K3双抗性突变株,产辅CoQ10的量达到157.7mg.L-1,比原始菌株提高了88.1%,并具有良好的遗传稳定性。结论所建方法简便可行。

OBJECTIVE To select CoQ10 highly producing strains.METHODSThe strain SY-3 which could produce CoQ10 was irradiated respectively by UV,LD-laser or both of them to increase CoQ10 production.RESULTSThe coordinative effect of UV and LD-laser was more ideal than using them alone.Initially,the cells were treated by UV for 120 seconds and then by LD-laser for 8 minutes.A CoQ10 highly producing mutant HR-99(NaN3r VK3r) was obtained and its productivity reached 157.7 mg·L-1,which were 88.1% higher that those of the ...