壳聚糖/海藻酸钠自组装微球的制备及释药性能

Preparation of Chitosan/alginate microspheres by layer-by-layer self-assembly method and in vitro drug release

目的利用壳聚糖(CS)聚阳离子及海藻酸钠(ALG)聚阴离子电解质的性质,在药物微球表面自组装形成多层包覆结构的壳聚糖载药微球,并研究组装层数、温度及盐离子浓度对自组装微球释药性能的影响。方法采用乳化交联法制备CS载四环素(TC)的药物微球,并在其表面交替自组装ALG及CS。利用IR测试技术及电极电位法进行表征。结果CS交联微球未破坏CS及TC的结构,CS与ALG以静电作用相结合。CS交联微球的载药量为40.2%,自组装六层的微球载药量为32%。组装后,药物释放时间延长,初期暴释现象得到极大改善,释药速率随组装层数的增加而下降,温度较高时组装完整,盐离子浓度存在较佳点。结论温度为60℃、盐离子浓度为0.5 mol.L-1、组装层数为四层的微球释药性能较佳。

OBJECTIVE Chitosan(CS) and alginate(ALG) were cation and anion polyelectrolyte respectively.Layer-by-layer(LbL) self-assembly technique was used to prepare tetracycline(TC) microspheres coated with CS and ALG.The effects of the layer number,deposition temperature and NaCl concentration on the release property of microspheres were investigated.METHODS CS microspheres loaded with TC were prepared by crosslinking-emulsion method.CS and ALG were absorbed on the CS/TC microspheres via LbL self-assembly technique...