影响出芽短梗霉XYE-7木聚糖酶生物合成的条件

The Biosynthesis of Xylanase by Aureobasidium pulluans XYE-7

类酵母真菌出芽短梗霉XYE-7是从果园土壤中分离到一株产木聚糖酶活力较高的菌株,对其合成木聚糖酶的条件进行了研究,结果表明该菌合成木聚糖酶的最佳培养基为:硫酸铵0.5%、酵母膏0.2%、磷酸二氢钾0.1%、硫酸镁0.05%、木聚糖0.5%,初始发酵pH值为2.0,发酵温度35℃,发酵时间为80h。在该条件下XYE-7合成木聚糖酶的能力最强。

Yeast-like strain XYE-7 for xylanase production was isolated from soil. The best carbon sources and nitrogen sources for the fermentation were as follows: 0.5% xylan, 0.5% (NH4)SO4, 0.2% barmene and 0.1% KH2PO4. The optimal initial pH value, culture temperature and fermentation time were 2.0, 35 ℃ and 80 h, respectively.