银杏叶提取物对猫局灶性脑缺血再灌注后神经细胞凋亡的影响

The Effection of GbE on The Apoptosis in Cats After Focal Cerebral Ischemia-repufution

目的:通过观察猫脑缺血再灌注后脑组织神经细胞凋亡现象,探讨银杏叶提取物的治疗保护作用。方法:健康家猫30只,随机分为2组,缺血对照组和银杏叶提取物治疗组。采用左侧眼眶入路制作大脑中动脉缺血再灌注模型。于缺血再灌注后第1天、第7天,采用Philip评分标准进行神经功能缺损评分;磁共振T2WI测定最终脑梗死体积,TUNAL法进行凋亡细胞检测,并进行免疫阳性细胞计数。结果:第7天时,治疗组与对照组比较神经功能受损程度轻,最终脑梗死体积小,凋亡细胞数目少,其差异具有统计学意义(P<0.05)。结论:银杏叶提取物能够减少凋亡细胞数目,促进神经功能修复,对急性脑梗死具有显著治疗保护作用。

Objective:To study the apoptosis of brain on the cats after focal cerebral ischemia-repufution and explore the protective effect of GbE.Methods:30 healthy adult cats were equally devided into the contral group and GbE group at random.All animals were operated to establish the feline models of cerebral focal ischemia--reperfusion by the approach of left transorbital.The neurological defects score were evaluated after 1d and 7d by Philips criteria score.The final infraction volum were evalued by T2WI on MR im...