高产木聚糖酶菌株的筛选及其产酶特性研究

Selection of high xylanase yielding bacterium strain and its enzyme producing property

寻找一种新的产木聚糖酶菌并从整体上降低木聚糖酶的生产成本.本试验以湖南岳阳某厂的土壤为样品,利用透明圈比较法和DNS法相结合,筛选出26株产木聚糖酶菌,并从中分离、纯化出一株高产木聚糖酶菌—S6,通过对S6菌株的发酵条件和产酶规律的试验,结果表明:S6菌株产酶的最优条件是以自制玉米半纤维素为碳源、添加0.2%硝酸铵、pH值为5.5浓度为0.01mol/L的柠檬酸-磷酸二氢钠缓冲体系的培养基中,28℃培养75h,菌生长进入对数期,酶活最大,达到156.2U/mL。

Taking sample from Hunan Yueyang Pulp and Paper-made Factory and Yueyang Tree Cultivation Factory as raw materials,a strain of high xylanase yielding bacteria was screened and purified from 26 strains of xylanase yielding bacteria by means of clearing halo comparison and DNS.Results:Optimal fermentation condition and property of enzyme producing were investigated,experimental results showed that the optimal fermentation condition of S6 is as follows:using the corn as the sole carbon,adding 0.2% NH4NO3 with ...