人类黑质神经元衰老性变化的体视学研究

Stereological Analysis of Age Associated Neuronal Changes in Human Substantia Nigra

目的探讨人类中脑黑质神经元的增龄性变化规律。方法选取19例正常人尸检的中脑组织,分为青年组(17～35岁)、中年组(40～59岁)和老年组(60～84岁),取中脑黑质连续冰冻切片,进行焦油紫染色和酪氨酸羟化酶(TG)免疫组化染色,用体视学计数原理及相应分析系统进行计数,观察老化过程中黑质神经元的形态学改变规律。结果随着年龄增长,人类中脑黑质神经元总数和黑色素性神经元均减少(P<0.05),其中以酪氨酸羟化酶阳性且带有神经黑色素的多巴胺能神经元数量减少较为突出,差异有统计学意义(P<0.05)。结论神经黑色素(NM)可能参与并促进了衰老过程中黑质神经元的退行性变和丢失,这可能是黑质病变的原因之一。

Objective To survey the regularities of dopamine neurons in substantia nigra(SN) of middle brain with age increasing and further provide objective evidence for revealing lesion of substantia nigra.Methods Nineteen normal individuals were divided into young(17 to 35 years old),middle-aged(40 to 59 years old),and aged(60 to 84 years old) groups.The age-related changes of neuromelanin(NM) containing and tyrosine hydroxylase(TH) immunoreactive cell number of SN were analyzed.Results Stereological counting based...