盐酸氟西汀胶囊含量的高效液相色谱法测定

HPLC determination of fluoxetine hydrochloride capsules

目的建立测定氟西汀含量的高效液相色谱法。方法选用Agilent Eclipse XDB-C_8柱(4.6mm×250 mm,5μm);Lab Alliance C_8保护柱(4.6 mm×10 mm,5μm);流动相:甲醇-水=62∶38;流速:1.0 mL·min^(-1);检测波长:226nm;进量样:20μL;柱温:室温。结果本法简便、准确、快速。线性范围:1.0~400μg·mL^(-1)(r=0.9997)。回收率在98.4~105.8%之间,RSD在0.68~2.52%(n=3)。结论方法利于氟西汀胶囊的质量控制。

OBJECTIVE To establish HPLC method for the determination of fluoxetine hydrochloride capsules.METHOD Agilent Eclipse XDB-C_8 column(4.6 mm×250 mm,5μm),with the mobile phase consisted of methanol-water(62:38),detection wavelength at 226 nm and external standard method.RESULTS The average recovery of artemether was 98.4~105.8%,with RSD of0.68~2.52%(n=3).CONCLUSION The method is simple,rapid and the results is accurate and reliable.