盐酸丁卡因注射液含量测定方法的改进

The improvement of method of content determination for Tetracaine hydrochloride Injection

目的寻找一种快速测定盐酸丁卡因注射液含量的方法。方法根据丁卡因在311nm处有最大吸收的特点,用紫外分光光度法测定其含量。结果以水为空白,在190~400hm范围内扫描,盐酸丁卡因在311nm处有最大吸收,并在每mL含2μg^20μg范围内呈良好的线性关系,直线方程为A=0.07540C—1.6164×10^(-4),r=0.9999,RSD=0.27%,并与《中国药典》法和《中国医院制剂规范》法比较,结果无显著差异,(P<0.01)。结论用紫外分光光度法测定盐酸丁卡因注射液的含量,操作简便,结果可靠,适于医院制剂快速分析。

OBJECTIVE To find a method of determination Tetracaine hydrochloride Injection quickly.METHODS Tetracaine Hydrochloride can be determined by Auto-Polarimeter,detection wavelength was 311 nm.RESULTS The concentration of Tetracaine hydrochloride was liner with optical activity in the range of 2μg/mL ~ 20μg/mL,r = 0.9999,RSD = 0.27%,re = 8.CONCLUSIONS Tetracain Hydrochloride Injection can be determined by polarimetry,The method was rapid and accurate.It was suitable for the quickanalysis of drugs made in hospital.