不同浓度NAA和KT对马铃薯组培苗的影响及方程模型

Effect of NAA and KT on Potato Seedlings in Tissue Culture and the Model Functions Establishment

研究了不同浓度的NAA和KT对马铃薯组培苗鲜重、根重、须根数和腋芽数的影响。结果表明,不同浓度的NAA和KT对马铃薯组培苗的各项指标的影响是不相同的,且二者有交互作用。构建了不同浓度的 NAA和KT影响鲜重、根重、须根数和腋芽数的模型方程。并计算出鲜重最大时二者的浓度配比为NAA:KT =0．0649:0．6020,根重最大时二者的浓度配比为NAA:KT=0．0904:0．4260,须根数最多时二者的浓度配比为NAA:KT=0．0728:0．51 93,腋芽数最多时二者的浓度配比为NAA:KT=0．0561:0．2420(单位均为mL／L)。综合考虑,最佳的浓度配合NAA为0．05-0．08 mL/L,KT为0．4-0．6 mL/L。

Effect of different concentration of NAA and KT on the fresh weight, root weight, root number, bud number of pototo tissue culture seedlings were studied The results showed that different concentration of NAA and KT has various influence on the potato seedling The model functions had also been established, they were Zfrcs weight = - 15. 3442x2-0. 1004xy-0. 1204y2 + 2. 0513x + 0. 1515 y+0. 148, Zroot weight =-1. 8812x2-0. 1123xy-0. 02398y2 + 0. 3881x + 0. 0305y + 0. 032, Zrootnumber = -537. 77x2-4.3989xy-2. 2983y2 + 80. 558x + 2. 7072y + 3. 8, Zbud number = -121. 553x2-4. 6841 xy - 0. 9933y2+14. 769x + 0. 7435y + 2. For fresh weight, the best concentration of NAA and KT were 0. 0649 ml/L and 0. 6020 ml/L,for root weight, the best concentration of NAA and KT were 0. 0904 ml/L and 0. 4260 ml/L,for root number, the best concentration of NAA and KT were 0. 0728 ml/L and 0. 5193 ml/L,for bud number, the best concentration of NAA and KT were 0. 0561 ml/L and 0. 2420 ml/L. So the best concentration of NAA and KT were 0. 05 - 0. 08 ml/L and 0. 4 - 0. 6 ml/L for potato tissue culture seedlings.