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survivin基因启动子驱动的肿瘤特异性腺病毒载体的构建 被引量：2

Construction of a tumor specific adenoviral vector piloted by survivin promoter
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摘要 目的:构建肿瘤特异性启动子驱动的腺病毒载体,检测survivin基因启动子在喉癌细胞中的特异表达活性。方法:PCR扩增survivin启动子,构建分别携带survivin启动子和CMV启动子的真核表达载体pCMV-EGFP和pSurp-EGFP,体外连接法制备重组腺病毒Ad-SP-EGFP和Ad-CMV-EGFP,后转染Hep-2细胞及血管内皮细胞ECV304,荧光显微镜下观察EGFP表达。结果:成功构建survivin基因启动子的腺病毒载体;Ad-SP-EGFP转染后荧光镜下见Hep-2细胞发出较强的绿色荧光,而ECV304细胞无表达。结论:成功制备肿瘤特异性survivin启动子驱动的腺病毒,为进一步开发肿瘤的特异性基因治疗奠定了实验基础。 Objective:To construct adenoviral vector piloted by tumor specific promoter and to detect the specific expression of survivin gene promoter in laryngocarcinoma cells.Method:The survivin gene promoter was generated by PCR to produce the eukaryotic expression vector pCMV-EGFP and pSurp-EGFP carrying with CMV promoter or survivin promoter.The original recombinant recombinant viruses Ad-SP-EGFP and Ad-CMV-EGFP were harvested by ligation in vitro.The two adenovirus were transfected into Hep-2 cell and vessel end...
作者 白万胜 成诗银 王军利 卞卡 张惠中 刘丽
机构地区 第四军医大学唐都医院耳鼻咽喉科 第四军医大学唐都医院临床实验科
出处 《陕西医学杂志》 CAS 北大核心 2008年第8期959-961,共3页 Shaanxi Medical Journal
基金 国家自然科学基金资助项目(No:30572024)
关键词 喉肿瘤 基因疗法 @腺病毒载体 @survivin基因 Laryngeal neoplasms Gene therapy @Adenoviral vector @Survivin gene
分类号 R739.65 [医药卫生—肿瘤]
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参考文献10

  • 1[1]Nettelbeck DM,Jerome V,Muller R.Gene therapy:designer promoters for tumor targeting.Trends Genet,2000,16(4):174-181.
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同被引文献22

  • 1曹美菊,赵惠东,赵君庸.中药材的硒含量及硒作用的影响因素[J].陕西中医,1994,15(12):559-562. 被引量：4
  • 2孙兰萍,张胜义,许晖.硒化壳聚糖的制备及理化性质的研究[J].食品工业科技,2006,27(2):145-146. 被引量：36
  • 3Kamihira S,Yamada Y,Hirakata Y,et al.Aberrant expression of caspase cascade regulatory gene in adult T-cell Lenkaemia:Survivin is an important determinant for prognosis.BrJ Haematol,2001,114(1):63-69.
  • 4Shin S,Sung BJ,Cho YS,et al.An anti-apoptotic protein human surviving is a direct inhibitor of caspase-3 and -7.Biochemistry,2001,40(4):1117-1123.
  • 5Ambrosini G, Adida C, Ahieri D C. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma[J]. Nat Med, 1997,3(8) : 917 -921.
  • 6Li F, Ambrosini G, Chu E Y, et al. Control of apoptosis and mitotic spindie checkpoint by survivin[J]. Nature, 1998, 396(6711): 580-584.
  • 7Li F. Survivin study: what is the next wave? [J]. J Cell Physiol, 2003, 197(1) : 8 -29.
  • 8Ryan B M, O'Donovan N, Duffy M J. Survivin: a new target for anticancer therapy[ J ]. Cancer Treat Rev, 2009, 35 (7) : 553 - 562.
  • 9Nettelbeck D M, Jerome V, Muller R. Gene therapy: designer promoters for tumour targeting[ J]. Trends Genet, 2000, 16(4) : 174 - 181.
  • 10Lo H W, Day C P, Hung M C. Cancer-specific gene therapy[ J]. Adv Genet, 2005, 54 : 235 - 255.

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陕西医学杂志
2008年 第8期

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