EB病毒感染对人胃上皮细胞GES-1增殖的影响

Effect of EBV Infection on the proliferation of Gastric Epithelial Cell GES-1

目的:探讨EB病毒感染人胃上皮细胞GES-1后对GES-1增殖的影响。方法:用携带新霉素抗性(NEOr)基因的重组EBV以密切接触法感染人胃上皮细胞系GES-1,同时以正常人胃上皮细胞GES-1为对照。经G418筛选出EBV感染阳性克隆,进行细胞形态学检测,并采用免疫组织化学染色法测定EBNA1的表达以鉴定EBV感染细胞克隆。对上述细胞用细胞计数法及MTT法检测细胞增殖情况;流式细胞术对细胞周期进行分析。结果:感染EBV的GES-1细胞大部分为EBNA1表达阳性,通过G418筛选,获得了稳定的EBV感染细胞克隆。EBV感染组细胞体积变大,核浆比增加。细胞计数法及MTT法绘制细胞生长曲线显示细胞增殖速度明显加快。流式细胞分析结果显示EBV感染组细胞S期延长。结论:EBV感染后使GES-1细胞稳定且高表达EBNA1,并且能够促进GES-1细胞的增殖。

Objective:To analyze the relationship between the infection of EBV and the proliferation of human gastric epithelial cell GES-1.Methods:In this experiment,recombined EBV Akata 1061 carrying neomycin resistance gene(NEOr)was used to infect GES-1 by intimate contact method,in the mean time the normal GES-1 cell was used as control.And then,the geneticin 418(G418)was adopted to select positive clones in EBV infected cells.The morphologic changes of GES-1 cells were observed under optical microscope.The method ...