雷公藤多甙对HaCaT细胞NF-κB的影响

Effects of polyglycoside of Tripterygium wilford ii HookF on NF-kappa B expression in HaCaT cells

目的:探讨雷公藤多甙(TWP)对HaCaT细胞核转录因子-κB(NF-κB)表达的影响。方法:体外培养人角质形成细胞株HaCaT细胞,设0.1μg/ml、0.5μg/ml、2.5μg/mlTWP组、空白对照组、P物质组。用上述药物预处理24h后,加入10-5mol/L的P物质作用30min,采用免疫组化、蛋白印迹和逆转录-聚合酶链反应法分别检测HaCaT细胞NF-κB阳性细胞表达率、NF-κB蛋白和mRNA表达情况。P物质组和空白组分别加入10-5mol/LP物质和细胞培养液。结果:空白组可见少量阳性细胞和一定量蛋白和mRNA表达。P物质组NF-κB阳性细胞表达率、蛋白和mRNA表达水平与空白组相比均明显升高;与P物质组相比2.5μg/ml雷公藤多甙组阳性细胞表达率、蛋白和mRNA水平均下降。而0.1μg/ml和0.5μg/ml雷公藤多甙组细胞NF-κB阳性细胞表达率、蛋白和mRNA表达水平与P物质组间无明显差异,且高于空白对照组。结论:小剂量的雷公藤多甙对HaCaT细胞核转录因子的表达无明显影响,大剂量的雷公藤多甙可下调HaCaT细胞的NF-κB的表达。

Objective:To study Tripterygium wilford ii HookF(TWP)potency on the expression of nuclear factor-kappa B(NF-κB)in HaCaT cells.Methods:Human benign epidermal keratinocytes(HaCaT cells)were cultured with different concentration of TWP(0.1μg/ml,0.5μg/ml and 2.5μg/ml)for 24 h,then 10-5 mol/L substance P was added to the cells.After 30 min,NF-κB expression rate in the cells was observed by immunocytochemistry,NF-κB P65 protein expression was evaluated by Western-blot,and mRNA expression was evaluated by reverse ...