摘要
目的:探讨黄芪组分(黄芪皂苷和黄芪多糖)对心肌细胞能量代谢的干预作用及其分子机制,为中药防治心衰提供实验依据。方法:体外培养的乳鼠心肌细胞,加血管紧张素Ⅱ(AngⅡ)造成肥大心肌细胞模型,黄芪组分配伍干预后,采用RT-PCR法测定肌酸激酶(CK)同功酶的mRNA表达。结果:1.AngⅡ作用24 h后,CK-B亚基的表达增加而CK-M亚基的表达减少,48 h该作用持续存在;2.黄芪不同组分及配伍干预48 h,心肌细胞的CK-B亚基及CK-M亚基表达趋于正常。结论:黄芪组分及组分配伍可在分子水平上抑制AngⅡ对心肌细胞CK同功酶mRNA表达的影响,提示黄芪可能是通过对肥大心肌细胞能量代谢的干预作用预防心衰的发生发展。
Objective:To observe whether Radix Astragali saponins and Astragali polysaccharides can effectively interfere with the cellular energy metabolism and its mechanism and to provide scientific evidence for the application of the Chinese herbs preventing and treating congestive heart failure.Methods:mRNA expression of isoenzyme creatine kinase(iso-CK) was investigated by means of RT-PCR,based on the study of the effect of Radix Astragali saponins and Astragali polysaccharides,through the cultured neonatal myocy...
出处
《中国实验方剂学杂志》
CAS
2008年第5期40-43,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金资助项目(No:30772693)
教育部博士点专项基金资助项目(20050026004)
关键词
黄芪
血管紧张素Ⅱ
心肌肥大
细胞培养
Radix Astragali
angiotensin Ⅱ
cardiac hypertrophy
cellar culture
