凉膈丸定性定量方法研究

Studies on Qualitative and Quantitative Methods of Liangge Pills

目的:凉膈丸的定性定量方法研究。方法:采用TLC法对制剂中的黄芩、栀子进行定性鉴别;用HPLC对制剂中的大黄药材进行含量测定。色谱柱:Alltima ODS C18柱(4.6 mm×250 mm,5μm);流动相:甲醇-乙腈-0.2%磷酸(40∶40∶20);流速:1.0 mL.min-1;检测波长为254nm。结果:TLC法能对黄芩、栀子进行专属性定性分析;大黄酸在(0.055 86~0.558 6)μg范围内线性良好,r=0.999 6,平均回收率100.79%,RSD=1.04%(n=6);大黄素在(0.079 8~0.798 0)μg范围内线性良好,r=0.999 7,平均回收率102.64%,RSD=1.53%(n=6);大黄酚的含量在(0.063 84~0.638 4)μg范围内线性良好,r=0.999 8,平均回收率103.20%,RSD=0.82%(n=6)。结论:所建立的方法可准确的进行定性和定量检测,方法可靠,重复性好,可有效控制凉膈丸的质量。

Objective:To study the qualitative and quantitative methods of Liangge Pills.Methods: TLC was used to identify Radix Scutellariae,Fructus Gardenia;HPLC was used to determine rheinic acid,emodin and chrysophanol in the preparation.The samples were separated on an Alltima ODS C_(18)(4.6 mm × 250 mm,5 μm) column with methanol-acetonitril-0.1% phosphoric(40:40:20) as the mobile phase.The detection wavelength was set at 254 nm and the flow rate was 1.0 mL·min^(-1).Results: Radix Scutellariae,Fructus Gardeniae co...