三叶虫茶中白杨素苷的鉴定及定量分析

Identifier and Determination of Chrysin in San-Ye-Chongcha

目的:鉴定和测定三叶虫茶中的白杨素苷。方法:经丙酮提取、聚酰胺柱色谱分离、重结晶纯化,利用化学鉴别、UVI、R、LC-ESI/MS/MS对白杨素苷进行鉴定;HPLC定量测定,Gemini C18柱,流动相∶乙腈-水(30∶70),流速:1.0 mL.min-1,柱温:40℃,检测波长:270 nm。结果:从三叶虫茶中提取分离得白杨素-7-o-葡萄糖苷晶体,HPLC归一化法测得其纯度达99.2%;在(2~20)μg.min-1范围内,线性回归方程为:Y=7.739×104X-6.0×103(r=0.999 3),三批三叶虫茶中白杨素苷的平均含量为2.77%,平均回收率为97.3%,RSD为1.3%(n=5)。结论:首次从三叶虫茶中分离纯化得到白杨素-7-葡萄糖苷,且用HPLC法测定了其含量,测定结果准确、重现性好。

Objective:To identify and quantitatively determine chrysin-7-o-glucoside in Sanyechongcha.Method:The compunds were extracted with acetone and purified with polyamide column and recrystallization.The chemical structure was identified by chemical method,UV,IR and ESI/LC-MS/MS.The quantitative analysis was performed on a Gemini C_(18) column with acetonitrile-water(30∶70)as the mobile phase at flow rate of 1.0 mL·min^(-1).The detection wavelengh was at 270 nm.Result:The main ingredient with a purity of 99.2% w...