HPLC法测定痛经膏贴中黄芩苷和汉黄芩素含量研究

Determination of Baicalin and Wogonin in Tongjing Patch by HPLC

目的:探索HPLC测定痛经膏贴中黄芩苷和汉黄芩素含量的方法。方法:Zorbax SB-C18(4.6mm×250mm,5μm)色谱柱;流动相:甲醇-水-磷酸(58:42:0.05);流速:1.0mL.min-1;检测波长:278nm。结果:黄芩苷的线性范围为(0.44～2.20)μg(r=0.9997),平均回收率为98.64%,RSD=1.17%;汉黄芩素的线性范围为(0.0128～0.2048)μg(r=0.9999),平均回收率为98.96%,RSD=2.08%;3批样品中黄芩苷和汉黄芩素的含量分别为7.33%、6.74%、7.29%和0.71%、0.65%、0.63%。结论:测定方法简便、准确,可同时测定痛经膏贴中黄芩苷和汉黄芩素含量。

Objective:To develop an HPLC method to determine the contents of both baicalin and wogonin in Tongjing Patch.MethodA Zorbax SB-C18 column(4.6×250 mm,5 μm)was used.The mobile phase was composed of methanol-water-acetic acid(58∶42∶0.05) and the flow rate was 1.0 ml·min-1.UV detection wave length was set at 278 nm.Results:The linear range of baicalin was(0.44～2.20)μg(r= 0.999 7)and the average recovery was 98.64% and the RSD was 1.17%.The linear range of wogonin was(0.012 8 ～ 0.204 8)μg(r= 0.999 9)and the aver...