摘要
目的:观察乳腺癌耐药蛋白(breast cancer resistance protein,BCRP)、谷胱甘肽-S-转移酶-π(GST-π)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达,探讨其与化疗药物敏感性的关系。方法:应用MTT法对60例未经化疗的NSCLC患者行体外化疗药物多柔比星、拓扑替康、紫杉醇、长春新碱和顺铂的敏感性检测,并用免疫组织化学方法对BCRP、GST-π在NSCLC中的表达进行检测,将其表达情况与对应的化疗耐药性进行相关分析。结果:60例NSCLC患者中,31例(51.7%)对多柔比星耐药,32例(53.3%)对拓扑替康耐药,12例(20.0%)对紫杉醇耐药,15例(25.0%)对长春新碱耐药,32例(53.3%)对顺铂耐药。腺癌耐药例数多于鳞癌,但两者差异无统计学意义,P>0.05。BCRP、GST-π在60例NSCLC患者中阳性率分别为43.3%(26/60)和60.0%(36/60),BCRP、GST-π在腺癌中的阳性率高于鳞癌,但两者差异无统计学意义,P>0.05。BCRP和GST-π在NSCLC中的表达呈明显相关,P<0.05。BCRP和GST-π的表达与化疗药物敏感性之间均存在明显相关,P<0.05。结论:BCRP、GST-π可能共同参与介导NSCLC的多药耐药,两者在NSCLC中的阳性表达及共表达可作为预测NSCLC患者多药耐药的指标之一。
OBJECTIVE: To observe the expressions of BCRP and GST-π in non-small cell lung cancer and investigate the relationship between the expressions and the drug sensitivity.METHODS: Sixty cases of NSCLC were tested with MTT assay to evaluate their chemoresistance to adriamycin,topotecan,paclitaxel,vincristine and cisplatin.The expressions of BCRP and GST-π were examined with the immunohistochemical assay,and then the relationship between the expressions of the two proteins above mentioned and the chemoresistance...
出处
《中华肿瘤防治杂志》
CAS
2008年第12期897-900,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
陕西省科学技术研究发展计划项目(2004K13-G17)
