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S100分子结合域缺失对钙周期结合蛋白生物学功能影响的研究 被引量：4

Effect of S100 binding domain on biological behavior of CacyBP/SIP
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摘要 目的:探讨S100结合域的缺失对钙周期素结合蛋白(CacyBP/SIP)生物学功能的影响。方法:构建野生型CacyBP/SIP及S100结合域截短突变的真核表达载体(分别为pFLAG-CacyBP和pFLAG-△S100),脂质体法转染至人胃癌细胞系MKN45,四唑盐(MTT)比色法观察细胞生长,平板克隆实验及裸鼠成瘤实验检测转染细胞的体外和体内成瘤性。结果:成功构建了野生型CacyBP/SIP及△S100截短突变的真核表达载体并转染MKN45细胞。野生型CacyBP/SIP及△S100截短突变的CacyBP/SIP均能抑制胃癌细胞MKN45的增殖,使MKN45细胞的体外及体内成瘤性降低,但是截短突变CacyBP/SIP抑制胃癌细胞增殖及成瘤性的作用更强。结论:CacyBP/SIP能够抑制胃癌细胞的恶性表型,而阻断S100分子与CacyBP/SIP的结合能增强CacyBP/SIP这种抑制作用。 OBJECTIVE:To explore the effect of S100 binding domain on the biological behavior of CacyBP/SIP.METHODS:The eukaryotic expression vectors of the wild-type CacyBP/SIP and truncated mutant lacking S100 binding domain required for association with S100 proteins were constructed and transfected into gastric cancer cell line MKN45 by using liposome.The cell growth was determined by MTT,and plate clonogenic assay and tumor xenograft in nude mice were performed respectively to test tumorigenecity of the cells in v...
作者 宁晓暄 孙世仁 张坤 龚卫琴 张珊红 李源
机构地区 西安第四军医大学西京医院老年病科 西安第四军医大学西京医院肾脏内科 陕西师范大学生命科学院细胞生物学研究室
出处 《中华肿瘤防治杂志》 CAS 2008年第13期968-971,共4页 Chinese Journal of Cancer Prevention and Treatment
基金 国家自然科学基金(30572113) 西京医院科技创新基金(XJCX05M21)
关键词 胃肿瘤/病理学 钙结合蛋白质类/遗传学 S100蛋白质类 转染 stomach neoplasma/pathology calcium-binding proteins/genetics S100 proteins transfection
分类号 R735.2 [医药卫生—肿瘤]
  • 相关文献

参考文献9

  • 1[1]Marenholz I,Heizmann C W,Fritz G.S100 proteins in mouse and man:from evolution to function and pathology (including an update of the nomenclature)[J].Biochem Biophys Res Commun,2004,322 (4):1111-1122.
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  • 3宁晓暄,孙世仁,李源,龚卫琴,刘理礼,孙力,梁洁,潘阳林,程翌,吴开春,樊代明.钙周期素结合蛋白对胃癌细胞生长增殖的影响[J].中华医学杂志,2006,86(46):3264-3268. 被引量：1
  • 4[4]Filipek A,Jastrzebska B,Nowotny M,et al.CacyBP/SIP,a calcyclin and Siah-1-interacting protein,binds EF-hand proteins of the S100 family[J].J Biol Chem,2002,277 (32):28848-28852.
  • 5[5]Jastrzebska B,Filipek A,Nowicka D,et al.Calcyclin (S100A6) binding protein (CacyBP) is highly expressed in brain neurons[J].J Histochem Cytochem,2000,48 (9):1195-1202.
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二级参考文献11

  • 1Filipek A.S100A6 and CacyBP/SIP-two proteins discovered in Ehrlich ascites tumor cells that are potentially involved in the degradation of beta-catenin.Chemotherapy,2006,52:32-34.
  • 2Matsuzawa SI,Reed JC.Siah-1,SIP,and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses.Mol Cell,2001,7:915-926.
  • 3Shi Y,Hu W,Fan D,et al.Regulation of drug sensitivity of gastric cancer cells by human calcyclin-binding protein (CacyBP).Gastric Cancer,2004,7:160-166.
  • 4Filipek A,Wojda U.p30,a novel protein target of mouse calcyclin (S100A6).Biochem J,1996,320:585-587.
  • 5Filipek A,Jastrzebska B,Nowotny M,et al.CacyBP/SIP,a calcyclin and Siah-1-interacting protein,binds EF-hand proteins of the S100 family.J Biol Chem,2002,277:28848-28852.
  • 6Filipek A,Kuznicki J.Molecular cloning and expression of a mouse brain cDNA encoding a novel protein target of calcyclin.J Neurochem,1998,70:1793-1798.
  • 7Jastrzebska B,Filipek A,Nowicka D,et al.Calcyclin (S100A6)binding protein (CacyBP) is highly expressed in brain neurons.J Histochem Cytochem,2000,48:1195-1202.
  • 8Pircher TJ,Geiger JN,Zhang D,et al.Integrative signaling by minimal erythropoietin receptor forms and c-Kit.J Biol Chem,2001,276:8995-9002.
  • 9Zhao Y,Shi Y,Fan DM,et al.Differentially expressed gene profiles between multiple drug resistant gastric adenocarcinoma cells and their parental cells.Cancer Lett,2002,185:211-218.
  • 10Araki Y,Okamura S,Hussain SP,et al.Regulation of cyclooxygenase-2 expression by the Wnt and ras pathways.Cancer Res,2003,63:728-734.

同被引文献20

引证文献4

二级引证文献6

中华肿瘤防治杂志
2008年 第13期

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