中药清毒栓对宫颈癌SiHa细胞凋亡的影响

Effect of Qingdushuan on apoptosis of cervical cancer SiHa cell

目的探讨中药清毒栓对宫颈癌SiHa细胞增殖抑制及凋亡的作用。方法设立空白血清组及中药清毒栓组、保妇康栓组、西药安达芬组,以4%含药血清培养液作用于SiHa细胞,分为3个时间点,通过MTT法检测各组对细胞增殖抑制的影响;同时运用流式细胞分析技术进行TUNEL法检测晚期细胞凋亡的变化。结果经含药血清培养液作用后各组SiHa细胞数量减少,其中以作用72h时对细胞抑制作用最强,与空白血清组比较,差异有统计学意义(P<0.01),且抑制作用安达芬组>清毒栓组>保妇康组,清毒栓组与安达芬组间差异有统计学意义(P<0.05)。各含药血清组对细胞凋亡均有显著的影响,且明显优于空白血清组(P<0.05),各含药血清组凋亡率依次为清毒栓组>保妇康组>安达芬组,差异均有统计学意义(P<0.01)。结论清毒栓含药血清通过促进细胞凋亡而达到抑制肿瘤的目的。

Objective To study proliferation and apoptosis of cervical cancer treated by Qingdushuan in cell biology and molecular biology.Methods 4 groups(blank serum group,Qingdushuan group,Baofukang group and Andafen group)were developed,and the proliferation inhibiting effect of the drugs on AiHa cells(cultivated with 4%,containing serum)were detected by MTT assay.At the same time,apoptosis was detected using flow cytometry and TUNEL method.Results After cultured with medium containing drug,SiHa cells were signific...