摘要
选择16头天山马鹿种公鹿电刺激采精,通过探讨不同稀释液和甘油浓度等条件对精液冷冻处理的影响,拟建立一种天山马鹿精液冷冻的方法,并对冷冻精液品质和人工授精效果进行评定。结果表明:天山马鹿对电刺激采精有耐受性,麻醉保定的采精频率每周2次最佳,10月中旬精液品质最好;6%甘油的稀释液冷冻效果最优;细管冻精活率(48.14±13.15)%,顶体完整率(56.34±14.84)%;诱导同期发情和自然发情人工授精情期产羔率分别达到50.80%和78.50%。
Semen electroejaculated from 16 Tanshan wapiti stags and the different factors influencing their semen cryopreservation were explored.The factors include cryodiluent,freezing method and final concentration of glycerol.Based on the results,a new semen cryopreservation method has been developed,then motility,acrosome integrity and fertility of these frozen semen were evaluated.The results showed the best frequency of electroejaculation was 2 times each week.During the course of electroejaculation,voltage had ...
出处
《中国兽医学报》
CAS
CSCD
北大核心
2008年第7期858-861,共4页
Chinese Journal of Veterinary Science
基金
新疆维吾尔自治区高校科研计划科学研究重点资助项目(XJEDU2004I70)
新疆生产建设兵团塔里木畜牧科技重点实验室资助项目(200508)
关键词
天山马鹿
电刺激采精
精液冷冻
人工授精
Tianshan wapiti
electroejaculation
semen crypreservation
artificial insemination
