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应用流式细胞术检测脂肪肝综合征肉鸡肝细胞生长周期与凋亡

Detection of hepatocellular cycle and apoptosis of fatty liver syndrome (FLS) inbroilers by flow cytometry
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摘要 用高能低蛋白饲料饲喂1日龄三黄肉鸡7周,建立脂肪肝综合征(FLS)病理模型,以流式细胞术的方法检测肝细胞生长周期及细胞凋亡,以探讨FLS的发病机理。与对照鸡比较,5~7周龄试验鸡肝细胞S期、G2+M期和增殖指数(PI)降低,G0/G1期升高;肝细胞凋亡率4~7周龄显著升高(P<0.01)。结果表明,高能饲料在体内氧化,产生大量自由基,损伤肝细胞DNA,使肝细胞分裂增殖减缓并发生凋亡,同时低蛋白饲料缺少转运脂质的必要条件,脂肪堆积于肝细胞内,肝细胞的功能受损而发生FLS。 One-day old 3-Huang broilers were fed on the high-caloricity/low-protein diet for setting up pathological model of FLS to study hepatocyte cycle and apoptosis by Flow Cytometry.Compared with the control group,S and G2+M phases of the hepatocellular cycle was lower,and G0/G1 phase higher from 5 to 7 weeks in group B.Also,percentage of the hepatocellular apoptosis was markedly increased(P<0.01) from 4 to 7 weeks in group B.The results showed that free radical were markedly increased in FLS caused by the hi...
作者 吴强 彭西 李英伦 徐之勇 崔恒敏
机构地区 四川农业大学动物医学院
出处 《中国兽医学报》 CAS CSCD 北大核心 2008年第8期978-981,共4页 Chinese Journal of Veterinary Science
基金 四川省教育厅重点项目
关键词 脂肪肝综合征 高能低蛋白饲料 流式细胞术 细胞周期 细胞凋亡 肉鸡 fatty liver syndrome high-caloricity/low-protein flow cytometry cell cycle apoptosis broiler
分类号 S858.31 [农业科学—临床兽医学]
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参考文献9

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中国兽医学报
2008年 第8期

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