嗜线虫真菌Duddingtonia flagrans减少绵羊粪便中线虫感染性幼虫剂量测定

Dose titration of nematode-trapping fungus Duddingtonia flagrans in reducing in fective larvae from sheep faeces

嗜线虫真菌Duddingtonia flagrans是目前发现的一种最具有动物胃肠道线虫病生物防治应用潜力的真菌。为了解该菌捕杀绵羊粪便中感染性幼虫效果与剂量的关系,为今后该制剂应用和质量检验标准的制定提供依据,用不同剂量的厚垣孢子,分别以不经消化道直接加入感染羊粪便,或作添加剂通过饲喂进入消化道后采集直肠粪便,经培养检测感染性幼虫数量的变化。结果表明,嗜线虫真菌Duddingtonia flagrans具有良好的捕杀胃肠道线虫感染性幼虫的生物学特性。以制剂厚垣孢子4×103/g剂量加入感染羊粪便,或以每天5×105/kg体质量的剂量饲喂绵羊,可使粪便培养物中线虫感染性幼虫数减少83.6%~87.5%。

Nematode-trapping fungus Duddingtonia flagrans is found to be the most potential strain in biological contral of animal nematode diseases so far.To evaluate the relationship of dosage between efficacy in trapping infective larvae from sheep faeces and to provide criterion for clinic usage and the establishment of stands for quality inspection,different doses of chlamydospores were mixed with infected sheep faeces directly,or feed infected sheep as additive daily and faecese were collected before feeding.The...