小鼠包虫病动物模型的建立

Establishment of Laboratory Animal Models for Hydatid disease

[目的]人工感染法建立包虫病动物模型。[方法]将藏羊源细粒棘球绦虫原头蚴悬液1ml(每ml悬液含4000个原头蚴,青霉素1000U,链霉素1000U)注射于50只(25♂♂25♀♀)4周龄ICR小鼠腹腔内,每周测体重与腰围1次;设空白对照组10只(5♂♂5♀♀)。[结果]接种第20天后,动物体重和腰围均高于空白对照组的各项指标;第90天剖检动物,包囊生成率为96%(48/50),人工感染成功率为96%。[结论]绵羊源性细粒棘球绦虫原头蚴悬液腹腔注射ICR小鼠建立包虫病动物模型的方法可为包虫病的防制研究工作提供简便、可靠和实用的实验动物模型。

[Objective] To establish laboratory animal models for hydatid disease. [Methods] 50 ICR(4 weeks old,25♂and 25♀) mice were inoculated in abdomen with 1 ml suspended liquid containing 4000 Echinococcus from Tibet sheep and 1000U Penicillin and 1000U Streptomycin. Examined body weight and waistline in every week to compare with Blank control. [Results] After 20 days, the body weight and waistline of experimental animals were higher than blank control animals. On the 90ths day , all of the animals were used to ...