摘要
研究了常压及高压条件下以豆饼粉、棉籽粉酸水解液作氮源,对链霉菌(Streptomyces)HS-1产转谷氨酰胺酶的影响．初步试验高压条件下以所得豆饼粉水解物作氮源,链霉菌(streptomyces)HS-1产转谷氨酰胺酶的酶活可达3．86μmol／(min·mL)．对高压条件下豆饼粉的水解条件进行确定：以3mol／L的盐酸水解4h所得水解物的发酵酶活较高,为5．17μmol／(min·mL),且进一步实验以蛋白胨与豆饼粉水解液作混合氮源时发酵酶活较单氮源高．
The effect of hydrochloric acid hydrolysis of soybean cake powder and cottonseed powder under different pressure were investigated on MTGase production by Streptomyces. The results showed that hydrolysate of soybean cake powder in high pressure served as favorable source. MTGase activity could reach 3. 86 μmol/(min·mL). The optimum of hydrolysis of soybean cake powder is followed:suitable concentration of hydrochloric acid is 3 mol/L,hydrodysis time is 4 hours. The experimental results showed that the most ...
出处
《华东师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第Z1期183-187,共5页
Journal of East China Normal University(Natural Science)
关键词
豆饼粉
酸水解
转谷氨酰胺酶
soybean cake powder
acid hydrolysis
MTGase
