摘要
从河北、北京、山东泰安和潍坊等地区的萝卜、大白菜、甘蓝、油菜上得到8个芜菁花叶病毒(Turnip mosaic virus, TuMV)分离物,测定了它们3’-末端cDNA片段的序列。根据衣壳蛋白基因(cp)核苷酸序列可以将这8个分离物与另外2 个TuMV分离物分为3组:泰安旧镇大白菜(JZBC)、甘蓝(JZGL)和萝卜(JZLB)分离物为一组;北京(BJILB)、潍坊萝卜分离物(WFLB)与引起萝卜红心病的TuMV分离物(WFLB99)为一组;泰安范镇、河北、潍坊(WFLB04)萝卜和泰安旧镇油菜 (JZYC)上的TuMV分离物为一组。农壳蛋白氨基酸序列比较结果与此基本一致,所不同的是分离物WFLB99与所有分离物的差异均较大,形成单独一个分支。有必要对TuMV的变异情况和致病机理进行深入研究。
8 Turnip mosaic virus (TuMV) isolates were collected from radish, Chinese cabbage, cabbage and rape in Hebei Province, Beijing, Tai an and Weifang of Shandong Province. Their 3 -terminal genomic sequences were cloned and determined in the study. The analysis results of nucleotide sequences of their coat protein genes with those of other 2 TuMV isolates showed that they could be clustered into three groups. Three isolates, Chinese cabbage (JZBC), cabbage (JZGL) and radish (JZLB) from the same field in Jiuzhe...
出处
《植物病理学报》
CAS
CSCD
北大核心
2005年第S1期38-42,共5页
Acta Phytopathologica Sinica
基金
国家自然科学基金资助项目(30100117)山东省优秀中青年科学家奖励基金(2004BS06002)
关键词
芜菁花叶病毒
萝卜
衣壳蛋白
系统树分析
Turnip mosaic virus
radish
coat protein
phylogenetic analysis
