玉米新月弯孢菌(Curvularia lunata)的RAPD分析

RAPD analysis among Isolates of Curvularia lunata collected from corn in China

对分离自玉米弯孢菌叶斑病标样中的77株新月弯孢菌和1株来自水稻的新月弯孢菌进行RAPD分析表明,菌株间具有丰富的遗传多样性,在相似系数约0．60处,所有菌株被聚为3个组,但88．0％的菌株聚入第Ⅰ组内,其余菌株被聚入另外2个组内。第Ⅰ组内共有69个菌株,包含来自不同区域的致病性较强的菌株,是玉米弯孢菌叶斑病的优势类群,其余 2个类群主要是一些致病能力较弱或不致病的菌株。结果表明,新月弯孢菌种内菌株的遗传多样性与致病性相关,但与菌株地理来源无明显的直接关系。

78 isolates of Curvularia lunata, mainly Collected from corn Curvularia leaf spot in different geographic regions in China, were characterized by random amplified polymorphic DNA (RAPD) with 8 arbitrarily primers. There was extensive genetic diversity within these isolates. At similar level of 0. 60, all isolates were clustered into three distinct groups. 88. 0% isolates were assigned to groupl, the dominant group, whereas 12.0% isolates to the other two groups. The dominant group included most of the virul...