生防菌株HL29的鉴定及其抑菌活性研究

Identification and antagonistic activity of a biocontrol strain HL29

从形态、生理生化、16S rRNA三个方面确定了HL29的分类地位。光学显微镜下观察到菌体为杆状细胞,革兰氏染色均匀,并可见芽孢染成蓝紫色。扫描电子显微镜进一步观察到细胞壁外带有厚薄均一的黏质层,可见椭圆型芽孢,无伴胞晶体。生理生化测定结果表明,HL29在各项测试中与枯草芽孢杆菌的标准菌株表现一致;16S rRNA基因序列测序结果表明,HL29与Bacillus subtilis的同源性达到99．52％。抑菌活性测定结果表明,HL29菌株及代谢产物对包括三个亚门真菌10个属的14种植物病原真菌,以及4个重要属的代表性植物病原细菌均有不同程度的抑菌作用,表明HL29菌株的菌体和代谢产物具有较高的抑菌活性和较广抑菌谱。

On the basis of morphological, physiological and biochemical characters as well as sequence analysis of 16SrRNA the biocontrol strain HL29 was determined. Under optics microscope, the strain was bacilliform, G+ ,stained evenly purple spore. Furthermore,evenly distributing slime layer was found around the organism with elliptical spore but without parasporal crystal under SEM observation. According to the physiological and biochemical characters HL29 strain was consistent with standard Bacillus subtilis stra...