RNAiFect^(TM)和SuperFect转染试剂对4种细胞活性的影响

The lnfluence on the four kinds of cells by adding RNAiFect^(TM) and super fect

在RNA干涉中,转染试剂的用量与siRNAs的量成正相关关系,但因转染试剂对细胞有一定的毒性作用,超过一定用量可以引起细胞形状改变、脱落、甚至死亡,直接影响着干涉的效率,所以确定转染试剂的优化用量,是RNA干涉试验中的重要环节.将不同用量的RNA iFectTM和SuperFect两种转染试剂分别加入鸡胚成纤维(CEF)细胞、293细胞、PK细胞和MDCK细胞中,在4h和24h后观察细胞的活力情况,比较了不同转染试剂对不同细胞的影响.结果表明:相同用量的两种转染试剂对293细胞、PK细胞和MDCK细胞的生长的影响远远小于对CEF细胞的影响;两种转染试剂用于外源小分子转染时,建议在293细胞、PK细胞和MDCK细胞上的用量不超过3u l;两种转染试剂对CEF细胞的毒性较大,不适合用于转染.

In the RNA interference,the dosage of siRNAs has positive correlativity with that of transfection reagent.However,transfection reagents have a great influence on the growth of cells,including cells falling off,morphological change,even dying,etc.,when the dosage of the transfection reagent exceeds to some extend.So it is a very important process to RNA interference that ascertaining the optimization dosage of transfection reagent.In the test,the author added different dosages of two transfection reagents R...