三七总皂甙注射液体外诱导HL-60细胞凋亡的研究

Study on HL-60 Cell Apoptosis Inducing in vitro by Panax Notoginseng Saponins Injection

目的观察三七总皂甙诱导人白血病细胞株HL-60细胞凋亡作用及机制。方法在体外细胞培养的基础上用200～1600μg/ml三七总皂甙处理HL-60细胞,光镜下观察细胞形态；用MTT法测细胞生长抑制率,流式细胞仪检测凋亡峰,免疫组化法检测p53和bcl-2凋亡相关基因的表达,采用真彩色图像分析仪定量分析。结果200～1600μg/ml三七总皂甙可抑制HL-60细胞生长,抑制率有剂量依赖性。细胞凋亡峰随药物浓度增大而增加。三七总皂甙800、1600μg/ml可使p53表达上调,bcl-2表达明显下降,表达改变有浓度依赖性。结论三七总皂甙在体外可抑制HL-60细胞生长,诱导其凋亡,其诱导凋亡的作用与凋亡相关基因p53表达上调、而抗凋亡基因bcl-2表达下调有关。

Objective To investigate the apoptosis of human leukemia cell line HL-60 induced by Panax No- toginseng Saponins(PNS)and its mechanism.Methods HL-60 ceils were cultured and treated with 200～1 600μg/ml PNS in vitro.The morphology of ceils was examined under light microscope.The inhibitory rate of cell growth was measured by MTT method.The apoptotic peak was detected by flow cytometric analysis,p53 expression was measured with immunohistochemical technique,and bcl-2 apoptosis related gene expression was quant...