摘要
目的探讨中药肠道泡腾栓剂治疗溃疡性结肠炎的免疫学疗效机制。方法实验分设正常对照、模型对照、空白泡腾栓、清肠泡腾栓、清肠栓、柳氮磺胺吡啶栓共6个组,ELISA法检测血清、结肠上清TNF-α仪、IL-13；免疫组化、原位杂交检测IL-8(mRNA)阳性表达。结果模型对照组血清TNF-α较正常对照组升高(P＜0．05),结肠黏膜IL-8(mRNA)阳性表达明显增强(P＜0．01)；清肠泡腾栓明显降低结肠上清TNF-α含量以及IL-8(mRNA)阳性表达,与模型组比较差异有显著性(P＜0．05,P＜0．01)；清肠泡腾栓对IL-13的调控作用不明显,与模型对照组比较差异无显著性(P＞0．05)。结论清肠泡腾栓通过抑制促炎细胞因子而对溃疡性结肠炎产生治疗作用。
Objective To observe the immunity therapeutic mechanism of Qingchang Effervescent Supposito- ry(QES)in treating ulcerative colotitis(UC).Methods Six groups were set up,i.e.the normal control,the mod- el,the blank effervescent suppository(BES),QES,sulfasalazine(SASP)suppository and Qingchang suppository gronps.Tumor necrosis factorα(TNF-α)and IL-13 in blood serum and colon supernate were tested by ELISA,and interleukin-8(IL-8)mRNA positive expression by immunohistological method and in situ hybridization.Res...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2005年第S1期49-52,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
上海市科委2003年博士发展基金
关键词
清肠泡腾栓
溃疡性结肠炎
TNF-Α
IL-8
IL-13
实验研究
Qingchang Effervescent Suppository
ulcerative colotitis
tumor necrosis factorα
interleukin-8
interleukin-13
experimental study
