摘要
目的观察决明子对实验性大鼠糖尿病肾病(diabetic nephropathy,DN)治疗作用,并探讨其可能机制。方法32只雄性SD大鼠随机分成4组,即正常对照组、模型组、低、高剂量决明子治疗组,每组8只。模型组及治疗组采用STZ 60mg/kg体重一次性腹腔注射,正常对照组注射等量枸椽酸缓冲液。治疗组予决明子按低剂量10 g·kg^(-1)·d^(-1)与高剂量50g·kg^(-1)·d^(-1)水煎灌胃,对照组及模型组喂予等量饮用水。各组疗程均为8周。分别采用酶联免疫吸附(enzyme-linked immunosorbent assay,EUSA)法测定各组大鼠外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中核因子-κB(nuclear factor kappa B,NF-κB)活性；免疫组化检测各组肾组织NF-κB、纤粘蛋白(fibronectin,FN)的表达；光镜观察肾组织形态。结果与模型组比较,决明子明显抑制NF-κB活化和FN表达,减少24h尿蛋白排泄,降低血脂及肌酐水平,减轻肾小球肥大、系膜细胞增生和细胞外基质堆积。结论决明子对DN具有明显的防治作用,抑制NF-κB的活化可能是其肾脏保护作用的机制之一。
Objective To observe the treatment of Cassia Seed (CS) on diabetic nephropathy of experimen- tal rats and to explore its possible mechanism.Methods Thirty-two male SD rats were randomly divided into 4 groups,i.e.the normal control,model,low dose of CS treated and high dose of CS treated group.Streptozotocin (STZ),60 mg/kg body weight,was once peritoneally injected to rats in the model and the treated groups,while equal volume of citric acid buffer liquid to rats in the normal control group.CS water decoctio...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2005年第S1期71-74,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
贵州省教委基金(F2000-5)
贵州省省长基金(S20001-8)
关键词
糖尿病肾病
大鼠
NF-KB
决明子
diabetic nephropathy
rats
nuclear factor kappa B
Cassia Seed
