摘要
文章对由肠膜状明串珠菌L.M-0326(Leuconostocmesenteriodes)发酵生产右旋糖酐的工艺过程及条件进行了探讨,通过对其发酵过程中的粘度、pH值、果糖、右旋糖酐生成和分子量变化的研究及对发酵诱导物的考察,得到了右旋糖酐发酵工艺的相关工程曲线。结果表明:通过定向发酵技术可得到符合要求的不同分子量的右旋糖酐,避免了老工艺中酸水解工艺;控制发酵培养基pH值为8.0,可使产率最大;无机盐离子Mn2+和Ca2+能促进L.M菌的生长,其促进菌生长的程度大小依次为:Mn2++Ca2+>Mn2+>Ca2+。
The process and condition in the preparation of dextran through the fermentation of Leuconostoc mesenteriodes-0326 were discussed in the paper.The adhesive viscosity,pH value, the production of fructose and dextran,variation of molecular-weight and the fermentation inducer were investigated,and the related curves of the dextran fermentation presented.The results indicate that different molecular-weight of dextran could be obtained by direct fermentation,and the acid hydrolyzing in the old technology be avoided.The yield reached the maximum by controlling the pH value to be 8.0,and the inorganic salt could promote the growth of the Leuconostoc mesenteriodes with the effect of promotion being Mn~~2++Ca~~2+>Mn~~2+>Ca~~2+.
出处
《合肥工业大学学报（自然科学版）》
CAS
CSCD
2004年第7期783-787,共5页
Journal of Hefei University of Technology：Natural Science
基金
安徽省经贸委技术创新项目([2001]265号文第128项)
合肥工业大学科研基金资助项目(040603F)
关键词
右旋糖酐
发酵
特性粘度
肠膜状明串珠菌
dextran
fermentation
inherent viscosity
Leuconostoc mesenteriodes
